The compound datasheet is excited by ultraviolet light at around 350 nm emits blue/ cyan fluorescence light around an emission maximum at 461 nm. Replicating or dividing chromosomes will not stain with Hoechst 33342 DNA dye. The technical data provided above is for guidance only. This product may be. Hoechst 33342 nucleic acid hoechst stain is a popular cell- permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. Biochim datasheet Biophys Acta 949, 158- 68. Portugal and Waring Biochim. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm.
1 It emits blue fluorescence ( excitation 350 nm/ emission maximum 461 nm) when bound to double stranded DNA is useful as a marker of nuclei for cell cycle studies to distinguish nuclear morphology in. HoechstNone hoechst None None None EU OEL hoechst ( STEL) EU Skin Notation hoechst Chemical Name Austria Denmark Poland Switzerland HoechstNone HoechstNone None None None None None Handling Chemical Name Ireland Norway Spain OEL ( TWA) HoechstNone None None Avoid contact with skin eyes clothing. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle datasheet studies in combination with BrdU. datasheet on our website. A comparative footprinting study. Hoechst- 33342 unlike other Hoechst dyes is datasheet more lipophillic due to an. Hoechst 33342 is a cell- permeable fluorescent compound ( MW 561. Storage: The lyophilized powder is stable for 1 year if kept below 0ºC with desiccant.
On binding to DNA, the fluorescence greatly increases. Hoechst datasheet 33342 is a specific fluorescent DNA stain that binds within the minor groove of hoechst double- stranded AT- rich regions. The BD Pharmingen™ Hoechst 33342 Solution is a reagent for the fluorescent staining of DNA nuclei in live fixed cells.
Hoechst 33342 is a cell- permeable fluorescent compound ( MW 561. 93) that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of. Hoechst 33342 emits blue ﬂ uorescence when bound to double stranded DNA. It is slightly more membrane per- meant than the Hoechst 33258 analog.
hoechst 33342 datasheet
Hoechst 33342 may be used to identify healthy or apoptotic nuclear mor- phology and for cell cycle studies. Each vial of Hoechst 33342 contains 1 mL of aqueous solution at 200 μg/ mL ( catalog # 639). Search results for hoechst 33342 at Sigma- Aldrich.